Envirolyte anolyte has been evaluated in independent microbiological tests and practical field applications for disinfection, sanitizing and water treatment purposes. This page summarises available evidence for bactericidal, yeasticidal and virucidal activity of anolyte generated by Envirolyte electrochemical systems.
Anolyte is an electrochemically activated disinfectant solution generated on site from water, salt and electricity. Depending on the operating parameters, it contains active chlorine species, including hypochlorous acid, which are responsible for its antimicrobial activity.
Original laboratory documents and test reports can be provided upon request together with the relevant Envirolyte equipment documentation.
Independent Microbiological Testing
Envirolyte systems and generated solutions have been tested in independent laboratory studies and used in on-site trials in hospitals, breweries, water systems, agriculture and other hygiene-sensitive environments.
The testing evidence helps customers evaluate Envirolyte technology for applications where microbial control, biofilm prevention, surface hygiene or water disinfection are important performance requirements.
For related technical information, see Electrolysed Water Solutions and Performance, Testing and Compliance Evidence .
Bactericidal Activity
In laboratory testing, the product under test, “Anolyte”, demonstrated bactericidal activity for hygienic handrub disinfection according to EN 13727:2012+A2:2015. The reported result was a ≥5 log reduction under clean conditions at 20.1 °C.
The test was performed using 250 ppm hypochlorous acid with a 60-second contact time against the following reference organisms:
- Pseudomonas aeruginosa
- Staphylococcus aureus
- Escherichia coli K12
- Enterococcus hirae
Yeasticidal Activity
Anolyte also demonstrated yeasticidal activity according to EN 13624:2013. The reported result was a ≥4 log reduction under clean conditions at 20.1 °C.
The test was performed using 250 ppm hypochlorous acid with a 60-second contact time against the reference strain Candida albicans.
This section should be described as yeasticidal activity rather than sporicidal activity unless a separate sporicidal test report is available.
Virucidal Activity
The antiviral activity of Anolyte was tested according to EN 14476, the quantitative suspension test method used to evaluate virucidal activity of chemical disinfectants and antiseptics in the medical area.
In the reported test, Anolyte in 1:1 dilution demonstrated virucidal activity against non-enveloped DNA and RNA viruses at 20 °C in the presence of 0.3 g/L BSA.
- ≥4 log reduction of Adenovirus type 5 after 1 minute contact time;
- ≥5.4 log reduction of Poliovirus 1 Sabin strain after 1 minute contact time;
- ≥4.2 log reduction of Murine Norovirus after 1 minute contact time.
According to the reported EN 14476 test interpretation, activity against adenovirus, poliovirus and murine norovirus supports a broad virucidal activity claim under the conditions of the test.
Published In-Vitro Research
Envirolyte also references published in-vitro research on electrolyzed water, including studies evaluating the effectiveness of anolyte or hypochlorous acid against different microorganisms and fungi species associated with healthcare and nosocomial environments.
An Investigation Into the In-Vitro Effectiveness of Electrolyzed Water
The following tables summarise the reported activity of Envirolyte® electrolyzed water against ATCC reference strains and clinical bacterial strains at different dilution rates and contact times.
| Microorganism | Time | 1/1 | 1/2 | 1/10 | 1/20 | 1/50 | 1/100 |
|---|---|---|---|---|---|---|---|
| Acinetobacter baumannii ATCC 19606 | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| E. coli ATCC 25922 | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | + | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| Enterococcus faecalis ATCC 29212 | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| Klebsiella pneumoniae ATCC 254988 | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| Pseudomonas aeruginosa ATCC 27853 | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| Staphylococcus aureus ATCC 29213 | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + |
min: minute; (−): no growth on culture; (+): bacteria growth on culture.
| Microorganism | Time | 1/1 | 1/2 | 1/10 | 1/20 | 1/50 | 1/100 |
|---|---|---|---|---|---|---|---|
| Acinetobacter baumannii | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| E. coli | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| Vancomycin resistant Enterococcus faecium | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| Klebsiella pneumoniae | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| Pseudomonas aeruginosa | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| Methicillin resistant Staphylococcus aureus | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| Bacillus subtilis | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | + | + | + | |
| 10 min | − | − | − | + | + | + | |
| 30 min | − | − | − | − | + | + | |
| Myroides spp. | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + |
min: minute; (−): no growth on culture; (+): bacteria growth on culture.
In-Vitro Efficiency of Electrolyzed Water Against Fungi Species
The following tables summarise the reported effects of Envirolyte® electrolyzed water on the growth of yeast and mold species frequently detected in nosocomial environments.
| Yeast | Time | 1/1 | 1/2 | 1/10 | 1/20 | 1/50 | 1/100 |
|---|---|---|---|---|---|---|---|
| C. albicans | 1 min | − | − | − | − | + | + |
| 2 min | − | − | − | − | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| C. tropicalis | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | + | + | + | |
| 10 min | − | − | − | + | + | + | |
| 30 min | − | − | − | − | + | + | |
| C. parapsilosis | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | + | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| C. glabrata | 1 min | − | − | − | − | + | + |
| 2 min | − | − | − | − | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | − | + | |
| 30 min | − | − | − | − | − | − | |
| C. krusei | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + | |
| C. lusitaniae | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | + | + | + | |
| 10 min | − | − | − | + | + | + | |
| 30 min | − | − | − | − | + | + | |
| Trichosporon spp. | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | + | + | + | |
| 10 min | − | − | − | + | + | + | |
| 30 min | − | − | − | − | + | + |
(−): growth was not observed; (+): growth was observed.
| Molds | Time | 1/1 | 1/2 | 1/10 | 1/20 | 1/50 | 1/100 |
|---|---|---|---|---|---|---|---|
| A. fumigatus | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | − | + | + | |
| 5 min | − | − | − | − | − | + | |
| 10 min | − | − | − | − | − | + | |
| 30 min | − | − | − | − | − | + | |
| A. flavus | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | − | + | + | |
| 5 min | − | − | − | − | − | + | |
| 10 min | − | − | − | − | − | − | |
| 30 min | − | − | − | − | − | − | |
| A. niger | 1 min | − | − | − | + | + | + |
| 2 min | − | − | − | + | + | + | |
| 5 min | − | − | − | − | + | + | |
| 10 min | − | − | − | − | + | + | |
| 30 min | − | − | − | − | + | + |
(−): growth was not observed; (+): growth was observed.
Typical Applications Supported by Microbiological Evidence
Microbiological testing data can support technical evaluation of Envirolyte systems for a wide range of disinfection and hygiene applications, including:- drinking water and process water disinfection;
- food and beverage processing hygiene;
- CIP and equipment sanitizing;
- healthcare and veterinary hygiene applications;
- agriculture, livestock and greenhouse hygiene;
- cooling towers and industrial water systems;
- Legionella prevention and microbial control programs.
Important Notes for Customers
Microbiological performance depends on the concentration of active substances, pH, temperature, organic load, contact time, target microorganisms and the surface or water conditions in the actual application. Customers should validate the selected Envirolyte system and operating parameters for their own process, country, regulatory requirements and intended use. For compliance-related information, see ECHA / BPR Information , Customer Requirements and Quality Control .Frequently Asked Questions
Available test information includes bactericidal testing against organisms such as Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli K12 and Enterococcus hirae, yeasticidal testing against Candida albicans, and virucidal testing against adenovirus, poliovirus and murine norovirus.
Log reduction is a way to express how much the number of microorganisms is reduced during a test. For example, a 4 log reduction corresponds to a 99.99% reduction under the defined test conditions.
Yes. Original documents can be provided upon request together with the relevant equipment or project documentation.
No. Laboratory results depend on defined test conditions. Real-world performance depends on concentration, contact time, water quality, organic load, temperature, system design and correct operation.
Request Microbiological Test Documentation
Envirolyte can provide microbiological testing information, technical documentation and project-specific recommendations for customers, distributors and OEM partners evaluating anolyte systems for disinfection, sanitizing or water treatment.
