This page summarises acute toxicity testing of Envirolyte anolyte using Daphnia magna, a standard aquatic test organism commonly used in ecotoxicological evaluation.
The test results provide useful information for customers evaluating Envirolyte anolyte for disinfection, sanitizing, water treatment and environmental applications. Results should always be interpreted according to the tested dilution, exposure time, test organism and actual application conditions.
Envirolyte provides this information for technical and regulatory reference only. It does not replace project-specific risk assessment, local regulatory review or application-specific validation.
Independent Acute Toxicity Testing
The acute toxicity study was undertaken by the Institute of Chemistry of Tallinn Technical University, Estonia.
The study included 24-hour acute toxicity testing with Daphnia magna according to Finnish standard SFS 5062.
Two anolyte types were evaluated:
- Anolyte medium at dilutions of 1:100, 1:500 and 1:1000;
- Anolyte strong at dilutions of 1:50, 1:200 and 1:500.
Acute Toxicity Test Results
The table below presents the reported EC50 values and the corresponding EC50 values calculated for the initial anolyte.
EC50: effective concentration causing a defined effect in 50% of the test organisms under the test conditions. “Not toxic” refers to the reported result for the specific dilution and test conditions.
Interpretation of the Results
The acute toxicity test demonstrated that no acute effects were reported for anolyte diluted 1:500 or more under the conditions of the study.
The test also reported no significant differences in acute toxicity between the two tested anolyte types, medium and strong, when results were recalculated for the initial anolyte.
These findings are important for customers considering Envirolyte anolyte in water treatment, process hygiene, agriculture, livestock, industrial systems and other applications where environmental discharge or dilution may be relevant.
Important Notes for Customers
Acute toxicity data should not be interpreted as a general statement that anolyte is non-toxic in every possible use case. Toxicity depends on concentration, dilution, exposure time, water chemistry, target organism, temperature, organic load and the discharge or application environment.
Customers should evaluate the selected Envirolyte system, generated anolyte concentration and discharge conditions for their own application and local regulatory requirements.
For related information, see Chronic Toxicity, By-products Testing, Microbiological Testing and Quality Control.
Typical Applications Where Acute Toxicity Data May Be Relevant
- drinking water and process water disinfection;
- agricultural and livestock water applications;
- cooling towers and industrial water systems;
- wastewater and effluent-related applications;
- food and beverage processing hygiene;
- greenhouse, horticulture and aquaculture-related projects.
The required dilution, monitoring and discharge controls should be confirmed for each project.
Frequently Asked Questions
The acute toxicity test used Daphnia magna, a small aquatic organism commonly used in ecotoxicity testing.
EC50 means the effective concentration at which a defined effect is observed in 50% of the test organisms under the specific test conditions.
No. According to the reported results, no acute effects were observed for anolyte diluted 1:500 or more under the conditions of the test.
No. Real-world results depend on concentration, dilution, exposure time, water chemistry, system design, operating parameters and local discharge requirements.
Envirolyte can provide available toxicological testing information and related technical documentation upon request for project evaluation, tenders or regulatory discussions.
Request Toxicological Documentation
Envirolyte can support customers, distributors and OEM partners with available acute toxicity information, technical documentation and project-specific data for evaluation of anolyte systems.
